Magi had performed the worship rituals in courts of kings; no sacrifice could be offered without a Magi, because Magi were the only way for relationship with gods. The issue of the present article is the studying of sacrifice ceremony and ritual during Achaemenid period and its impact on the enhancement of their power and influence during Ancient Iran. Therefore, the aim of this research is to answer this question based on comparative study according to classic texts and Persepolis fortification tablets that what was the Magi’s role especially in sacrifice ceremony and ritual during the Achaemenid period? The findings of this research shows that as Achaemenid kings had risen to power, the ceremony and ritual of sacrifice had held more glorious and therefore more quota and present had allocated to them. This issue resulted in the power for Magi, stabilization of their position and influence in Achaemenid state, formation of various ranks and also the raised of power in religious rank in religion and state of ancient Iran.